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Aktivering af real-time kompensation i hurtige fotokemiske oxidationer af proteiner til bestemmelse af Protein Topografi Ændringer
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Chapters
- 00:04Introduction
- 00:47Fast Photochemical Oxidations of Proteins (FPOP) Optical Bench Preparation
- 02:43FPOP Experiment
- 03:58Compensation Performance
- 05:45Results: Representative Adalimumab FPOP and Compensated Myoglobin FPOP in MES Buffer
- 06:51Conclusion
Hurtig fotokemisk oxidation af proteiner er en ny teknik til strukturel karakterisering af proteiner. Forskellige opløsningsmiddeltilsætningsstoffer og ligands har varieret hydroxyl radikale skyllevæske egenskaber. For at sammenligne proteinstrukturen under forskellige forhold kræves der en real-time kompensation af hydroxyl radikaler genereret i reaktionen for at normalisere reaktionsbetingelserne.
Tags
Real-time CompensationFast Photochemical OxidationsProtein Topography ChangesProtein ConfirmationProtein InteractionsBuffer CompositionSample PurityProtein ConcentrationDosimetry MethodDrug DevelopmentProtein StructureFPOP Optical BenchLaserInline DosimeterExcimer LaserMotorized StageHydrogen PeroxideFPOP Solution
