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Isolamento das células CD8+ T residentes em rim do rato para análise de citometria de fluxo
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis

Isolamento das células CD8+ T residentes em rim do rato para análise de citometria de fluxo

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6,172 Views

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06:07 min

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June 27, 2020

DOI:

10.3791/61559-v

DOI:
10.3791/61559-v

•

06:07 min
June 27, 2020

•

11 Views
, ,
1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine,University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital,Central South University

Summary

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Após a infecção viral, o rim abriga um número relativamente grande de células CD8+ T e oferece uma oportunidade de estudar células TRM não mucosas. Aqui, descrevemos um protocolo para isolar linfócitos renais de camundongos para análise de citometria de fluxo.

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Cite this Article

Liao, W., Ma, C., Zhang, N. Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis. J. Vis. Exp. (160), e61559, doi:10.3791/61559 (2020).

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