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Isolamento delle cellule CD8 + T residenti neireni del topo per l'analisi della citometria a flusso
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis
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Isolamento delle cellule CD8 + T residenti neireni del topo per l'analisi della citometria a flusso

italiano

Automatically Generated

6,172 Views

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06:07 min

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June 27, 2020

DOI:

10.3791/61559-v

DOI:
10.3791/61559-v

•

06:07 min
June 27, 2020

•

11 Views
, ,
1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine,University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital,Central South University

Summary

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A seguito di infezione virale, il rene ospita un numero relativamente elevato di cellule CD8+ T e offre l'opportunità di studiare le cellule T RM nonmucose. Qui descriviamo un protocollo per isolare i linfociti renali del topo per l'analisi della citometria del flusso.

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Cite this Article

Liao, W., Ma, C., Zhang, N. Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis. J. Vis. Exp. (160), e61559, doi:10.3791/61559 (2020).

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