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Pulmoner Ortamda İmmün Hücrelerin ve Proinflamatuar Mediatörlerin Karakterizasyonu
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Immunology and Infection
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JoVE Journal Immunology and Infection
Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment
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Pulmoner Ortamda İmmün Hücrelerin ve Proinflamatuar Mediatörlerin Karakterizasyonu

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3,127 Views

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09:00 min

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June 24, 2020

DOI:

10.3791/61359-v

DOI:
10.3791/61359-v

•

09:00 min
June 24, 2020

•

1 Views
, , ,
1Department of Microbiology, Immunology, and Cell Biology,West Virginia University, 2Department of Neuroscience,West Virginia University, 3Rockefeller Neuroscience Institute,West Virginia University

Summary

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Bu protokol, iskemik inmenin bir murin modeli olan geçici orta serebral arter tıkanıklığını takiben pulmoner ortamda immün hücre kompozisyonu, sitokin profili ve kemokin profilindeki değişiklikleri tanımlamak için flow sitometrinin kullanımını açıklamaktadır.

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Cite this Article

Monaghan, K. L., Farris, B. Y., Zheng, W., Wan, E. C. K. Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment. J. Vis. Exp. (160), e61359, doi:10.3791/61359 (2020).

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