Journal
/
Immunology and Infection
/
Caracterización de células inmunes y mediadores proinflamatorios en el ambiente pulmonar
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment
Please note that all translations are automatically generated. Click here for the English version.

Caracterización de células inmunes y mediadores proinflamatorios en el ambiente pulmonar

español

Automatically Generated

3,127 Views

•

09:00 min

•

June 24, 2020

DOI:

10.3791/61359-v

DOI:
10.3791/61359-v

•

09:00 min
June 24, 2020

•

1 Views
, , ,
1Department of Microbiology, Immunology, and Cell Biology,West Virginia University, 2Department of Neuroscience,West Virginia University, 3Rockefeller Neuroscience Institute,West Virginia University

Summary

Automatically generated

Este protocolo describe el uso de la citometría de flujo para identificar los cambios en la composición de las células inmunitarias, el perfil de citoquinas y el perfil de quimiocinas en el entorno pulmonar después de la oclusión transitoria de la arteria cerebral media, un modelo murino de accidente cerebrovascular isquémico.

Explore More Videos

Immune CellsProinflammatory MediatorsPulmonary EnvironmentIschemic StrokeFlow Cytometric AnalysisLung Immune Cell TypesCytokinesChemokinesWhole Body Transcardial PerfusionMultiplex Bead ArraysHomogenization BufferLung Cell Medium

Read Article

Cite this Article

Monaghan, K. L., Farris, B. Y., Zheng, W., Wan, E. C. K. Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment. J. Vis. Exp. (160), e61359, doi:10.3791/61359 (2020).

Download .ris file

Copy

Share Video

.

Copy