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Caratterizzazione di cellule immunitarie e mediatori proinfiammatori in ambiente polmonare
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Immunology and Infection
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JoVE Journal Immunology and Infection
Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment
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Caratterizzazione di cellule immunitarie e mediatori proinfiammatori in ambiente polmonare

italiano

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3,127 Views

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09:00 min

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June 24, 2020

DOI:

10.3791/61359-v

DOI:
10.3791/61359-v

•

09:00 min
June 24, 2020

•

1 Views
, , ,
1Department of Microbiology, Immunology, and Cell Biology,West Virginia University, 2Department of Neuroscience,West Virginia University, 3Rockefeller Neuroscience Institute,West Virginia University

Summary

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Questo protocollo descrive l'uso della citometria a flusso per identificare i cambiamenti nella composizione delle cellule immunitarie, nel profilo delle citochine e nel profilo delle chemochine nell'ambiente polmonare dopo occlusione transitoria dell'arteria cerebrale media, un modello murino di ictus ischemico.

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Cite this Article

Monaghan, K. L., Farris, B. Y., Zheng, W., Wan, E. C. K. Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment. J. Vis. Exp. (160), e61359, doi:10.3791/61359 (2020).

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