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Acyl-PEGyl Exchange Gel Shift Assay zur quantitativen Bestimmung der Palmitoylierung von Gehirnmembranproteinen
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Biochemistry
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JoVE Journal Biochemistry
Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins
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Acyl-PEGyl Exchange Gel Shift Assay zur quantitativen Bestimmung der Palmitoylierung von Gehirnmembranproteinen

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6,264 Views

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08:28 min

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March 29, 2020

DOI:

10.3791/61018-v

DOI:
10.3791/61018-v

•

08:28 min
March 29, 2020

•

9 Views
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1Department of Pharmacology,UC Davis School of Medicine

Summary

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Die Palmitoylierung beinhaltet die Umarbeitung einer 16-Kohlenstoff-Palmitat-Moiety zu Cysteinrückständen von Zielproteinen in reversibler Weise. Hier beschreiben wir einen biochemischen Ansatz, den Acyl-PEGyl-Austausch-Gel-Shift (APEGS)-Assay, um den Palmitoylierungszustand eines Proteins zu untersuchen, das für Maushirnlysate von Interesse ist.

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Cite this Article

Speca, D. J., Diaz, E. Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins. J. Vis. Exp. (157), e61018, doi:10.3791/61018 (2020).

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