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Detectie van eiwit s-acylation met behulp van Acyl-Hars Assisted Capture
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Biochemistry
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JoVE Journal Biochemistry
Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
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Detectie van eiwit s-acylation met behulp van Acyl-Hars Assisted Capture

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9,416 Views

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08:31 min

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April 10, 2020

DOI:

10.3791/61016-v

DOI:
10.3791/61016-v

•

08:31 min
April 10, 2020

•

15 Views
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1Department of Biochemistry and Molecular Biology,McGovern Medical School at UT Health, 2MD Anderson UT Health Graduate School

Summary

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Acyl-RAC (Acyl-Resin Assisted Capture) is een zeer gevoelige, betrouwbare en gemakkelijk uit te voeren methode om omkeerbare lipidemodificatie van cysteïneresiduen (S-acylation) in een verscheidenheid van biologische monsters op te sporen.

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Cite this Article

Tewari, R., West, S. J., Shayahati, B., Akimzhanov, A. M. Detection of Protein S-Acylation using Acyl-Resin Assisted Capture. J. Vis. Exp. (158), e61016, doi:10.3791/61016 (2020).

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