Journal
/
Immunology and Infection
/
Determinazione dell'efficienza dell'inibitore chimico contro la crescita intracellulare del Toxoplasma Gondii utilizzando un saggio di crescita basato su Luciferase
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay
Please note that all translations are automatically generated. Click here for the English version.

Determinazione dell'efficienza dell'inibitore chimico contro la crescita intracellulare del Toxoplasma Gondii utilizzando un saggio di crescita basato su Luciferase

6,879 Views

•

09:49 min

•

April 29, 2020

DOI:

10.3791/60985-v

DOI:
10.3791/60985-v

•

09:49 min
April 29, 2020

•

1 Views
, , , , ,
1Department of Biological Sciences,Clemson University, 2Eukaryotic Pathogens Innovation Center,Clemson University

Tags

Toxoplasma GondiiLuciferase-based Growth AssayChemical InhibitorDrug TargetCRISPR-Cas9Intracellular PathogenHigh-throughput ScreeningParasite Doubling TimeHuman Foreskin FibroblastsLuciferase SubstrateMicroplate ReaderNormalized Fold-changeLinear Regression

Read Article

Cite this Article

Key, M., Bergmann, A., Micchelli, C., Thornton, L. B., Millard, S., Dou, Z. Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay. J. Vis. Exp. (158), e60985, doi:10.3791/60985 (2020).

Download .ris file

Copy

Share Video

.

Copy