Journal
/
Biology
/
Isolamento dei cardiomiociti da cuori fissi per l'immunocitochimica e l'analisi dello ploidy
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis
Please note that all translations are automatically generated. Click here for the English version.

Isolamento dei cardiomiociti da cuori fissi per l'immunocitochimica e l'analisi dello ploidy

italiano

Automatically Generated

6,565 Views

•

08:41 min

•

October 07, 2020

DOI:

10.3791/60938-v

DOI:
10.3791/60938-v

•

08:41 min
October 07, 2020

•

9 Views
, ,
1Department of Integrative Biology and Physiology,University of Minnesota, 2Lillehei Heart Institute, Department of Medicine,University of Minnesota, 3Stem Cell Institute,University of Minnesota

Summary

Automatically generated

L'obiettivo di questo lavoro è quello di sviluppare un metodo per isolare in modo riproducibile i cardiomiociti dal cuore adulto e misurare il contenuto di DNA e la nucleazione.

Explore More Videos

CardiomyocytesIsolationImmunocytochemistryPloidy AnalysisFixed HeartsPerfusionCollagenaseDissociationFiltrationCentrifugation

Read Article

Cite this Article

Yücel, D., Solinsky, J., van Berlo, J. H. Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis. J. Vis. Exp. (164), e60938, doi:10.3791/60938 (2020).

Download .ris file

Copy

Share Video

.

Copy