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Detection of Total Reactive Oxygen Species in Adherent Cells by 2′,7′-Dichlorodihydrofluorescein Diacetate Staining
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Biochemistry
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JoVE Journal Biochemistry
Detection of Total Reactive Oxygen Species in Adherent Cells by 2′,7′-Dichlorodihydrofluorescein Diacetate Staining

Detection of Total Reactive Oxygen Species in Adherent Cells by 2′,7′-Dichlorodihydrofluorescein Diacetate Staining

27,043 Views

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04:53 min

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June 23, 2020

DOI:

10.3791/60682-v

DOI:
10.3791/60682-v

•

04:53 min
June 23, 2020

•

39 Views
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1Department of Biochemistry and Molecular Biology,University of New Mexico

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Reactive Oxygen SpeciesDCFH-DA StainingAdherent CellsFluorescence MicroscopyFluorescence Plate ReaderOxidative StressHCT116 Colorectal Cancer CellsFerrous SulfateDoxorubicinRIPA BufferBradford AssayFluorescence Intensity Quantification

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Kim, H., Xue, X. Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining. J. Vis. Exp. (160), e60682, doi:10.3791/60682 (2020).

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