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Isolement concomitant des astrocytes primaires et des microglies pour l’infection de parasite de Protozoa
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Immunology and Infection
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JoVE Journal Immunology and Infection
Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection
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Isolement concomitant des astrocytes primaires et des microglies pour l’infection de parasite de Protozoa

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7,477 Views

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09:34 min

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March 18, 2020

DOI:

10.3791/60680-v

DOI:
10.3791/60680-v

•

09:34 min
March 18, 2020

•

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1Centro de Terapia Celular e Molecular (CTCMol),Universidade Federal de São Paulo (UNIFESP), 2Departamento de Ciências Biológicas, Centro de Terapia Celular e Molecular (CTCMol),Universidade Federal de São Paulo (UNIFESP)

Summary

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L’objectif global de ce protocole est d’instruire comment extraire, maintenir et dissocier les cellules astrocytes murines et microglies du système nerveux central, suivies d’une infection par des parasites protozoaires.

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Cite this Article

Pacheco, A. d. O. L., Amaral, M. P., de Farias, I. S., Bottino, L. Z. M. F., Bortoluci, K. R. Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection. J. Vis. Exp. (157), e60680, doi:10.3791/60680 (2020).

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