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Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity

English

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12,059 Views

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06:16 min

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December 07, 2019

DOI:

10.3791/60622-v

DOI:
10.3791/60622-v

•

06:16 min
December 07, 2019

•

17 Views
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1Division of Cancer, Department of Surgery and Cancer,Imperial College London, 2Centre for Cancer and Inflammation, Barts Cancer Institute,Queen Mary University of London, 3German Center for Neurodegenerative Diseases (DZNE)

Summary

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This protocol describes the surgical generation of orthotopic pancreatic tumors and the rapid digestion of freshly isolated murine pancreatic tumors. Following digestion, viable immune cell populations can be used for further downstream analysis, including ex vivo stimulation of T cells for intracellular cytokine detection by flow cytometry.

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Spear, S., McNeish, I. A., Capasso, M. Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity. J. Vis. Exp. (154), e60622, doi:10.3791/60622 (2019).

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