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マウス脳における微小グリア密度、形態および末梢骨髄細胞浸潤解析のためのIBA1およびTMEM119を用いた免疫蛍光染色
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Immunology and Infection
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JoVE Journal Immunology and Infection
Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
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マウス脳における微小グリア密度、形態および末梢骨髄細胞浸潤解析のためのIBA1およびTMEM119を用いた免疫蛍光染色

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29,284 Views

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10:40 min

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October 27, 2019

DOI:

10.3791/60510-v

DOI:
10.3791/60510-v

•

10:40 min
October 27, 2019

•

119 Views
, , , , ,
1Axe Neurosciences,Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine,Université Laval, 3Integrated Program in Neuroscience,McGill University, 4Center for Brain Immunology and Glia (BIG),University of Virginia

Summary

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このプロトコルは、IBA1およびTMEM119の免疫蛍光コシャリンのための段階的なワークフローを記述し、ミクログリア密度、分布、および形態の分析に加えて、ならびにマウス脳組織における末梢骨髄細胞浸潤の分析を説明する。

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Cite this Article

González Ibanez, F., Picard, K., Bordeleau, M., Sharma, K., Bisht, K., Tremblay, M. Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain. J. Vis. Exp. (152), e60510, doi:10.3791/60510 (2019).

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