Journal
/
Biology
/
原原小鼠肝细胞的分离和3D胶原蛋白三明治培养，研究细胞骨架在胆汁形成中的作用
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
Please note that all translations are automatically generated. Click here for the English version.

原原小鼠肝细胞的分离和3D胶原蛋白三明治培养，研究细胞骨架在胆汁形成中的作用

English

Automatically Generated

10,788 Views

•

10:12 min

•

December 20, 2019

DOI:

10.3791/60507-v

DOI:
10.3791/60507-v

•

10:12 min
December 20, 2019

•

9 Views
, , ,
1Laboratory of Integrative Biology,Institute of Molecular Genetics of the Czech Academy of Sciences

Summary

Automatically generated

这里介绍的是一个协议，使用改良的胶原酶灌注技术从成年小鼠肝脏分离小鼠肝细胞。还描述了肝细胞在3D胶原蛋白夹层设置中的长期培养，以及细胞骨骼成分的免疫标签，以研究胆汁癌形成及其对治疗的反应。

Explore More Videos

3D Collagen Sandwich CulturePrimary Mouse HepatocytesBile Canalicular FormationCytoskeletonLiver IsolationHepatocyte IsolationIn Vitro StudiesPerfusionHeparinDMEMCollagen IPBSCell Culture

Read Article

Cite this Article

Korelova, K., Jirouskova, M., Sarnova, L., Gregor, M. Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro. J. Vis. Exp. (154), e60507, doi:10.3791/60507 (2019).

Download .ris file

Copy

Share Video

.

Copy