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利用膜成熟腺细胞聚合培养（MAAC）的人类成熟腺细胞的分离与培养
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)
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利用膜成熟腺细胞聚合培养（MAAC）的人类成熟腺细胞的分离与培养

English

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17,814 Views

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06:28 min

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February 13, 2020

DOI:

10.3791/60485-v

DOI:
10.3791/60485-v

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06:28 min
February 13, 2020

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37 Views
, ,
1Bioscience Metabolism, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D,AstraZeneca, 2The Lundberg Laboratory for Diabetes Research,University of Gothenburg, 3Wallenberg Centre for Molecular and Translational Medicine,University of Gothenburg

Summary

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膜成熟脂肪细胞聚集培养（MAAC）是培养成熟人类脂肪细胞的新方法。在这里，我们详细介绍了如何从人类脂肪中分离脂肪细胞，以及如何设置MAAC。

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Alexandersson, I., Harms, M. J., Boucher, J. Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC). J. Vis. Exp. (156), e60485, doi:10.3791/60485 (2020).

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