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Sporing Superparamagnetic Iron oksid-merket Mesenchymal stamceller bruker MRI etter intranasal levering i en traumatisk hjerneskade murine modell
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model
DOI:

10:03 min

November 21, 2019

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Chapters

  • 00:05Title
  • 01:06Superparamagnetic Iron Oxide (SPIO) nanoParticles labeling of Mesenchymal Stem Cells (MSC)
  • 01:44Controlled Cortical Impact (CCI) Injury
  • 04:04Intranasal MSC Delivery
  • 05:57In Vivo Magnetic Resonance Imaging (MRI)
  • 06:54MSC Tracking and Quantification
  • 08:03Results: Representative SPIO-Labeled MSC Detection and Tracking
  • 09:08Conclusion

Summary

Automatic Translation

Presentert her er en protokoll for ikke-invasiv mesenchymal Stem Cell (MSC) levering og sporing i en mus modell av traumatisk hjerneskade. Superparamagnetic jernoksid nanopartikler anvendes som en MRI-sonde (magnetisk resonans imaging) for MSC-merking og ikke-invasiv in vivo-sporing etter intranasal levering med sann tids MRI.

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