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Tek Molekülgözlem için DNA Origami Nanoyapılar üzerinde Dynein ve Kinesin Motor Toplulukları üretimi
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
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Tek Molekülgözlem için DNA Origami Nanoyapılar üzerinde Dynein ve Kinesin Motor Toplulukları üretimi

Türkçe

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6,402 Views

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08:09 min

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October 15, 2019

DOI:

10.3791/60369-v

DOI:
10.3791/60369-v

•

08:09 min
October 15, 2019

•

1 Views
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1Department of Biological Sciences,Smith College, 2Center for Microscopy and Imaging,Smith College

Summary

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Bu protokolün amacı, DNA origami nanoyapılar üzerinde moleküler motor toplulukları oluşturmak ve toplam iç yansıma floresan mikroskopisi kullanarak topluluk hareketliliğini gözlemlemektir.

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Cite this Article

Hu, J., Derr, N. D. Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation. J. Vis. Exp. (152), e60369, doi:10.3791/60369 (2019).

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