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Productie van Dynein en Kinesin motor ensembles op DNA origami nanostructuren voor observatie van enkelvoudige moleculen
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Biochemistry
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JoVE Journal Biochemistry
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
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Productie van Dynein en Kinesin motor ensembles op DNA origami nanostructuren voor observatie van enkelvoudige moleculen

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6,402 Views

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08:09 min

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October 15, 2019

DOI:

10.3791/60369-v

DOI:
10.3791/60369-v

•

08:09 min
October 15, 2019

•

1 Views
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1Department of Biological Sciences,Smith College, 2Center for Microscopy and Imaging,Smith College

Summary

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Het doel van dit protocol is het vormen van ensembles van moleculaire motoren op DNA origami nanostructuren en observeren het ensemble motiliteit met behulp van totale inwendige reflectie fluorescentiemicroscopie.

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Cite this Article

Hu, J., Derr, N. D. Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation. J. Vis. Exp. (152), e60369, doi:10.3791/60369 (2019).

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