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从一个成年小鼠脑半球分离区域特异性微胶质,进行深度单细胞RNA测序
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
从一个成年小鼠脑半球分离区域特异性微胶质,进行深度单细胞RNA测序
DOI:
10.3791/60347-v
•
09:49 min
•
December 03, 2019
•
Lu Zhou
,
Qingyun Li
1
Department of Neurobiology
,
Stanford University School of Medicine
Chapters
00:05
Title
00:43
Brain Region Dissection
01:43
Mechanical Tissue Dissociation
02:40
Myelin Removal
04:25
Staining for Fluorescence-activated Cell Sorting
05:18
Index FACS Sorting
05:46
Single-cell RNA-sequencing Library Preparation
07:17
Results: Isolation of Region-Specific Microglia for Deep Single-Cell RNA Sequencing
09:16
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们为从成年小鼠大脑半球的不同解剖区域分离微胶质提供方案,然后为全长转录体进行深度单细胞RNA测序的半自动库制备。该方法将有助于阐明微胶质在健康和疾病中的功能异质性。
Tags
Microglia
Single-cell RNA Sequencing
Brain Regions
Cell Isolation
Cell Separation
Dounce Homogenizer
Magnetic Separation
RNA Extraction
Library Preparation
Deep Sequencing
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