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Kwantificering van co-enzym A in cellen en weefsels
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Biochemistry
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JoVE Journal Biochemistry
Quantification of Coenzyme A in Cells and Tissues
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Kwantificering van co-enzym A in cellen en weefsels

Nederlands

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8,037 Views

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08:51 min

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September 27, 2019

DOI:

10.3791/60182-v

DOI:
10.3791/60182-v

•

08:51 min
September 27, 2019

•

13 Views
, , ,
1Department of Infectious Diseases,St. Jude Children’s Research Hospital

Summary

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Deze methode beschrijft de monstervoorbereiding uit gekweekte cellen en dierlijk weefsel, extractie en derivatisering van coenzym A in de monsters, gevolgd door hogedrukvloeistofchromatografie voor zuivering en kwantificering van het guarderivaten coenzym a door absorptie of fluorescentiedetectie.

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Cite this Article

Frank, M. W., Subramanian, C., Rock, C. O., Jackowski, S. Quantification of Coenzyme A in Cells and Tissues. J. Vis. Exp. (151), e60182, doi:10.3791/60182 (2019).

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