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Método de Electroporation para in vivo entrega de DNA plasmídeo no adulto zebrafish Telencephalon
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Developmental Biology
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JoVE Journal Developmental Biology
Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon

Método de Electroporation para in vivo entrega de DNA plasmídeo no adulto zebrafish Telencephalon

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9,452 Views

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06:02 min

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September 13, 2019

DOI:

10.3791/60066-v

DOI:
10.3791/60066-v

•

06:02 min
September 13, 2019

•

4 Views
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1Institute of Stem Cell Research,Helmholtz Zentrum München, 2Graduate School of Systemic Neurosciences,Ludwig Maximilian University, 3Department of Cell Biology and Anatomy, Biomedical Center,Ludwig Maximilian University, 4Munich Cluster for Systems Neurology (SyNergy)

Summary

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É apresentado aqui um método do Electroporation para a entrega do ADN do plasmídeo e a rotulagem da pilha ependymoglial no telencephalon adulto do zebrafish. Este protocolo é um método rápido e eficiente para visualizar e traçar células ependymogliais individuais e abre novas possibilidades para aplicar a eletroporação a uma ampla gama de manipulações genéticas.

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Durovic, T., Ninkovic, J. Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon. J. Vis. Exp. (151), e60066, doi:10.3791/60066 (2019).

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