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Isolatie van macrofaag subsets en stromale cellen van myocardiale monsters van mens en muis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
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Isolatie van macrofaag subsets en stromale cellen van myocardiale monsters van mens en muis

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7,366 Views

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07:25 min

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December 17, 2019

DOI:

10.3791/60015-v

DOI:
10.3791/60015-v

•

07:25 min
December 17, 2019

•

9 Views
,
1Department of Medicine,Washington University School of Medicine, 2Department of Immunology and Pathology,Washington University School of Medicine, 3Department of Developmental Biology,Washington University School of Medicine

Summary

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Hier gepresenteerd is een protocol om verschillende subgroepen van macrofagen en andere niet-immuuncellen van mens en muis myocardium te isoleren door het voorbereiden van een enkelvoudige celsuspensie door enzymatische spijsvertering. Gating schema's voor flow cytometrie gebaseerde identificatie en karakterisering van geïsoleerde macrofagen worden ook gepresenteerd.

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Cite this Article

Bajpai, G., Lavine, K. J. Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens. J. Vis. Exp. (154), e60015, doi:10.3791/60015 (2019).

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