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Usando células-tronco pluripotentes induzidas pelo homem para a geração de células T específicas de antígeno tumoral
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

Usando células-tronco pluripotentes induzidas pelo homem para a geração de células T específicas de antígeno tumoral

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9,925 Views

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10:57 min

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October 24, 2019

DOI:

10.3791/59997-v

DOI:
10.3791/59997-v

•

10:57 min
October 24, 2019

•

5 Views
, , , , , ,
1Surgery Branch,National Cancer Institute, NIH, 2Center for Cell-Based Therapy,National Cancer Institute, NIH, 3Laboratory of Immunology, Institute for Frontier Life and Medical Sciences,Kyoto University

Summary

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Este artigo descreve um método para gerar células-tronco pluripotentes induzidas por antígeno funcional do tumor derivadas de CD8αβ+ únicas células T positivas usando o sistema de cocultura OP9/DLL1.

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Cite this Article

Good, M. L., Vizcardo, R., Maeda, T., Tamaoki, N., Malekzadeh, P., Kawamoto, H., Restifo, N. P. Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells. J. Vis. Exp. (152), e59997, doi:10.3791/59997 (2019).

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