Journal
/
Biochemistry
/
Visualizando dinâmica sadrindo de receptor estéta de células t usando microscopia de folha de luz de rede
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Visualizando dinâmica sadrindo de receptor estéta de células t usando microscopia de folha de luz de rede

português

Automatically Generated

7,745 Views

•

09:24 min

•

January 30, 2020

DOI:

10.3791/59914-v

DOI:
10.3791/59914-v

•

09:24 min
January 30, 2020

•

1 Views
,
1Committee on Cancer Biology,The University of Chicago, 2Pritzker School of Molecular Engineering,The University of Chicago

Summary

Automatically generated

O objetivo deste protocolo é mostrar como usar microscopia de folha de luz de rede para visualizar quatro dimensões a dinâmica do receptor de superfície em células vivas. Aqui são mostrados receptores de células T em células CD4+ T primárias.

Explore More Videos

Four-dimensional ImagingLattice Light-sheet MicroscopyLive Cell ImagingReal-time TrackingMolecular DynamicsT-cell ReceptorAntigen-presenting CellsSample PreparationCell Labeling

Read Article

Cite this Article

Rosenberg, J., Huang, J. Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy. J. Vis. Exp. (155), e59914, doi:10.3791/59914 (2020).

Download .ris file

Copy

Share Video

.

Copy