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Visualiseren van Surface T-Cell Receptor Dynamics vierdimensionaal met behulp van Rooster Light-Sheet Microscopie
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Biochemistry
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JoVE Journal Biochemistry
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
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Visualiseren van Surface T-Cell Receptor Dynamics vierdimensionaal met behulp van Rooster Light-Sheet Microscopie

Nederlands

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7,745 Views

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09:24 min

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January 30, 2020

DOI:

10.3791/59914-v

DOI:
10.3791/59914-v

•

09:24 min
January 30, 2020

•

1 Views
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1Committee on Cancer Biology,The University of Chicago, 2Pritzker School of Molecular Engineering,The University of Chicago

Summary

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Het doel van dit protocol is om te laten zien hoe rooster Light-Sheet Microscopie te gebruiken om vierdimensionaal visualiseren oppervlakte receptor dynamiek in levende cellen. Hier worden T-celreceptoren op CD4+ primaire T-cellen weergegeven.

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Cite this Article

Rosenberg, J., Huang, J. Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy. J. Vis. Exp. (155), e59914, doi:10.3791/59914 (2020).

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