Journal
/
Immunology and Infection
/
Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

English

Automatically Generated

8,205 Views

•

11:34 min

•

July 26, 2019

DOI:

10.3791/59894-v

DOI:
10.3791/59894-v

•

11:34 min
July 26, 2019

•

6 Views
, , ,
1Program in Immunology,Sackler School of Graduate Biomedical Sciences, 2Department of Molecular Virology and Microbiology,Baylor College of Medicine, 3Department of Molecular Biology and Microbiology,Tufts University

Summary

Automatically generated

This protocol describes how to induce the differentiation of M cells in human stem cell-derived ileal monolayers and methods to assess their development.

Explore More Videos

M CellsIntestinal Stem CellsInduced DifferentiationIleal Enteroid MonolayersExtracellular MatrixTranswell MembranesEDTATrypsinCell DisaggregationSingle Cells

Read Article

Cite this Article

Fasciano, A. C., Blutt, S. E., Estes, M. K., Mecsas, J. Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers. J. Vis. Exp. (149), e59894, doi:10.3791/59894 (2019).

Download .ris file

Copy

Share Video

.

Copy