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Formação de esferóides da célula do ligamento periodontal humano em filmes de quitosana
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04:14 min
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June 19, 2019
DOI:
1Department of Periodontology, School & Hospital of Stomatology,Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 2Department of Periodontology,Dental Diseases Prevention & Treatment Center of Jiading District, 3College of Materials Science and Engineering,Tongji University
Summary
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Aqui, nós apresentamos protocolos de cultivo de esferoides da pilha do ligamento periodontal humano (PDL) por películas do quitosana. A cultura de esferoides celulares tridimensionais (3D) fornece uma alternativa ao sistema convencional da cultura do poliestireno da cultura do tecido (TCPs).
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Cite this Article
Yan, X., Ran, X., Xia, S., Yang, Y., Zhou, M., Yuan, C., Luo, L. Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films. J. Vis. Exp. (148), e59855, doi:10.3791/59855 (2019).
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