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04:14 min
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June 19, 2019
DOI:
1Department of Periodontology, School & Hospital of Stomatology,Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 2Department of Periodontology,Dental Diseases Prevention & Treatment Center of Jiading District, 3College of Materials Science and Engineering,Tongji University
Summary
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ここでは、ヒト歯周靭帯(PDL)細胞スフェロイドをキトサン膜で培養するプロトコルを紹介する。三次元(3D)細胞スフェロイドの培養は、従来の組織培養ポリスチレン(TCPS)培養システムに代わるものとなる。
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Cite this Article
Yan, X., Ran, X., Xia, S., Yang, Y., Zhou, M., Yuan, C., Luo, L. Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films. J. Vis. Exp. (148), e59855, doi:10.3791/59855 (2019).
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