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DNA-Sequenzerkennung durch DNA-Primase mit High-Throughput Primase Profiling
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Biochemistry
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JoVE Journal Biochemistry
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
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DNA-Sequenzerkennung durch DNA-Primase mit High-Throughput Primase Profiling

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8,520 Views

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08:04 min

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October 08, 2019

DOI:

10.3791/59737-v

DOI:
10.3791/59737-v

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08:04 min
October 08, 2019

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7 Views
, , , , ,
1Department of Chemistry,Ben-Gurion University of the Negev, 2Center for Genomic and Computational Biology, Department of Biostatistics and Bioinformatics,Duke University

Summary

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Proteinbindende Mikroarray -Experimente (PBM) in Kombination mit biochemischen Assays verknüpfen die Bindungs- und katalytischen Eigenschaften von DNA-Primase, einem Enzym, das RNA-Primer auf Vorlagen-DNA synthetisiert. Diese Methode, die als High-Throughput Primase Profiling (HTPP) bezeichnet wird, kann verwendet werden, um DNA-bindende Muster einer Vielzahl von Enzymen zu offenbaren.

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Cite this Article

Ilic, S., Cohen, S., Afek, A., Gordan, R., Lukatsky, D. B., Akabayov, B. DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling. J. Vis. Exp. (152), e59737, doi:10.3791/59737 (2019).

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