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Charakterisierung von Proteinen durch Größen-Ausschluss-Chromatographie gekoppelt an Multi-Angle Light Scattering (SEC-MALS)
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
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Charakterisierung von Proteinen durch Größen-Ausschluss-Chromatographie gekoppelt an Multi-Angle Light Scattering (SEC-MALS)

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51,127 Views

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10:00 min

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June 20, 2019

DOI:

10.3791/59615-v

DOI:
10.3791/59615-v

•

10:00 min
June 20, 2019

•

92 Views
, , ,
1Wyatt Technology Corporation, 2Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science,The Hebrew University of Jerusalem, 3Danyel Biotech Ltd.

Summary

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Dieses Protokoll beschreibt die Kombination von Größenausschlusschromatographie mit Multi-Winkel-Lichtstreuung (SEC-MALS) zur absoluten Charakterisierung von Proteinen und Komplexen in Lösung. SEC-MALS bestimmt das Molekulargewicht und die Größe reiner Proteine, nativer Oligomere, Heterokomplexe und modifizierter Proteine wie Glykoproteine.

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Cite this Article

Some, D., Amartely, H., Tsadok, A., Lebendiker, M. Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS). J. Vis. Exp. (148), e59615, doi:10.3791/59615 (2019).

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