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Nükleazsız Oksijen Çöpçü Protocatechuate 3,4-Dioksijenaz İfadesi ve Saflaştırma
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Biochemistry
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JoVE Journal Biochemistry
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
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Nükleazsız Oksijen Çöpçü Protocatechuate 3,4-Dioksijenaz İfadesi ve Saflaştırma

Türkçe

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6,196 Views

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10:14 min

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November 08, 2019

DOI:

10.3791/59599-v

DOI:
10.3791/59599-v

•

10:14 min
November 08, 2019

•

6 Views
, , ,
1Cancer Biology and Genetics, College of Medicine,The Ohio State University

Summary

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Protocatechuate 3,4-dioxygenase (PCD) enzimatik substrat protokatechuic asit kullanarak sulu bir sistemden serbest diatomik oksijen kaldırabilirsiniz (PCA). Bu protokol, bu oksijen atma enziminin ekspresyonu, saflaştırılması ve aktivite analizini açıklar.

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Cite this Article

Messer, R. K., Lopez Jr., M. A., Senavirathne, G., Yoder, K. E. Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase. J. Vis. Exp. (153), e59599, doi:10.3791/59599 (2019).

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