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Expression und Reinigung von Nuklease-freier Sauerstofffänger Protocatechuate 3,4-Dioxygenase
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Biochemistry
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JoVE Journal Biochemistry
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
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Expression und Reinigung von Nuklease-freier Sauerstofffänger Protocatechuate 3,4-Dioxygenase

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6,195 Views

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10:14 min

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November 08, 2019

DOI:

10.3791/59599-v

DOI:
10.3791/59599-v

•

10:14 min
November 08, 2019

•

6 Views
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1Cancer Biology and Genetics, College of Medicine,The Ohio State University

Summary

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Protocatechuate 3,4-Dioxygenase (PCD) kann mit seinem Substrat Protocatechuic Säure (PCA) enzymatisch freien diatomischen Sauerstoff aus einem wässrigen System entfernen. Dieses Protokoll beschreibt die Expressions-, Reinigungs- und Aktivitätsanalyse dieses Sauerstoff-Aufräumenzyms.

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Messer, R. K., Lopez Jr., M. A., Senavirathne, G., Yoder, K. E. Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase. J. Vis. Exp. (153), e59599, doi:10.3791/59599 (2019).

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