Journal
/
Cancer Research
/
毛細血管電気泳動質量分析によるメタボロミクス解析のための培養付着細胞からの水性代謝産物の抽出
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
Please note that all translations are automatically generated. Click here for the English version.

毛細血管電気泳動質量分析によるメタボロミクス解析のための培養付着細胞からの水性代謝産物の抽出

日本語

Automatically Generated

7,878 Views

•

11:39 min

•

June 09, 2019

DOI:

10.3791/59551-v

DOI:
10.3791/59551-v

•

11:39 min
June 09, 2019

•

2 Views
, , , , , ,
1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory,National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center,National Cancer Center

Summary

Automatically generated

この記事の目的は、メタボロミクス分析のために培養した付着細胞からの水性代謝産物の抽出のためのプロトコルを記述することであり、特に、毛細管電気泳動-質量分光法。

Explore More Videos

Keyword Extraction:MetabolomicsCapillary Electrophoresis-mass SpectrometryCEMSAqueous MetabolitesCultured Adherent CellsCancer CellsMetabolite ExtractionBiomarkersChemotherapy ResponseCell CultureTrypsin-EDTACell SuspensionCentrifugation

Read Article

Cite this Article

Maruyama, A., Kami, K., Sasaki, K., Sato, H., Sato, Y., Tsuchihara, K., Makinoshima, H. Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry. J. Vis. Exp. (148), e59551, doi:10.3791/59551 (2019).

Download .ris file

Copy

Share Video

.

Copy