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Proliferasyon ınsan antijeni spesifik CD4+ T hücrelerinin Karboksflorcein Succinimidyl Ester kullanarak ölçülmesini
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester
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Proliferasyon ınsan antijeni spesifik CD4+ T hücrelerinin Karboksflorcein Succinimidyl Ester kullanarak ölçülmesini

Türkçe

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8,152 Views

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07:00 min

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June 04, 2019

DOI:

10.3791/59545-v

DOI:
10.3791/59545-v

•

07:00 min
June 04, 2019

•

13 Views
, , ,
1Immunology and Diabetes Unit,St. Vincent’s Institute of Medical Research, 2Department of Medicine,University of Melbourne, St. Vincent’s Hospital

Summary

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Burada sunulan bir protokol Proliferasyona CD4+ T hücreleri antijenik proteinler veya peptidler boya seyreltme kullanarak yanıt olarak. Bu tahlil nadir antijen özgü T hücrelere özellikle duyarlıdır ve antijen özgü hücrelerin klonlama kolaylaştırmak için değiştirilebilir.

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Cite this Article

Di Carluccio, A. R., Tresoldi, E., So, M., Mannering, S. I. Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester. J. Vis. Exp. (148), e59545, doi:10.3791/59545 (2019).

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