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İzotropik hafif levha mikroskobu ve otomatik hücre Lineage analizleri kataloğa Caenorhabditis elegans Embriyogenesis Subcellular çözünürlük ile
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June 06, 2019
DOI:
1Department of Neuroscience and Department of Cell Biology,Yale University School of Medicine, 2Developmental Biology Program,Sloan Kettering Institute, 3Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling,University of Connecticut Health Center, 4Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering,National Institutes of Health, 5WormGUIDES.org, 6Instituto de Neurobiología, Recinto de Ciencias Médicas,Universidad de Puerto Rico
Summary
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Burada, nörogelişme sırasında tek hücreli dinamikleri anlamak için C. elegans embriyoları yaşayan yüksek çözünürlüklü mikroskobik, Hesaplamalı araçlar ve tek hücreli etiketleme kullanarak bir Kombinatoryal yaklaşım sunuyoruz.
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Duncan, L. H., Moyle, M. W., Shao, L., Sengupta, T., Ikegami, R., Kumar, A., Guo, M., Christensen, R., Santella, A., Bao, Z., Shroff, H., Mohler, W., Colón-Ramos, D. A. Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution. J. Vis. Exp. (148), e59533, doi:10.3791/59533 (2019).
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