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Developmental Biology

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等方性光シート顕微鏡と自動細胞系譜分析による、細胞内分解能を Caenorhabditis elegans 胚発生

JoVE Journal
Developmental Biology

This content is Free Access.

JoVE Journal Developmental Biology

Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

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等方性光シート顕微鏡と自動細胞系譜分析による、細胞内分解能を Caenorhabditis elegans 胚発生

7,860 Views

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08:16 min

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June 06, 2019

DOI:

10.3791/59533-v

DOI:

10.3791/59533-v

•

08:16 min

June 06, 2019

•

12 Views

Leighton H. Duncan^1,5, Mark W. Moyle^1,5, Lin Shao^1,5, Titas Sengupta^1,5, Richard Ikegami^1,5, Abhishek Kumar^4,5, Min Guo^4,5, Ryan Christensen^4,5, Anthony Santella^2,5, Zhirong Bao^2,5, Hari Shroff^4,5, William Mohler^3,5, Daniel A. Colón-Ramos^1,5,6

¹Department of Neuroscience and Department of Cell Biology,Yale University School of Medicine, ²Developmental Biology Program,Sloan Kettering Institute, ³Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling,University of Connecticut Health Center, ⁴Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering,National Institutes of Health, ⁵WormGUIDES.org, ⁶Instituto de Neurobiología, Recinto de Ciencias Médicas,Universidad de Puerto Rico

Summary

ここでは、 elegans胚における高分解能顕微鏡、計算ツール、および単一細胞標識法を用いて、neurodevelopment 中の単一細胞動態を理解するための組合せアプローチを提示する。

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