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Isotrope licht-vel microscopie en geautomatiseerde Cell Lineage analyses catalogus Caenorhabditis embryogenese met subcellulaire resolutie
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June 06, 2019
DOI:
1Department of Neuroscience and Department of Cell Biology,Yale University School of Medicine, 2Developmental Biology Program,Sloan Kettering Institute, 3Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling,University of Connecticut Health Center, 4Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering,National Institutes of Health, 5WormGUIDES.org, 6Instituto de Neurobiología, Recinto de Ciencias Médicas,Universidad de Puerto Rico
Summary
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Hier presenteren we een combinatie benadering met behulp van hoge-resolutie microscopie, computationele tools, en single-cell etikettering in levende C. de embryo's van de neurologische om de enige cel dynamiek te begrijpen.
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Duncan, L. H., Moyle, M. W., Shao, L., Sengupta, T., Ikegami, R., Kumar, A., Guo, M., Christensen, R., Santella, A., Bao, Z., Shroff, H., Mohler, W., Colón-Ramos, D. A. Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution. J. Vis. Exp. (148), e59533, doi:10.3791/59533 (2019).
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