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丝状真菌曲霉菌的单步富集蛋白脱乙酰化酶的酶活性测定
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
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丝状真菌曲霉菌的单步富集蛋白脱乙酰化酶的酶活性测定

English

Automatically Generated

6,484 Views

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09:07 min

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May 01, 2019

DOI:

10.3791/59527-v

DOI:
10.3791/59527-v

•

09:07 min
May 01, 2019

•

2 Views
, , , ,
1Division of Molecular Biology, Biocenter,Medical University of Innsbruck, 2Biology Department,Maynooth University, 3Maynooth University Human Health Research Institute

Summary

Automatically generated

像 RpdA 这样的1级组蛋白去乙酰化酶 (Hdac) 作为治疗真菌感染的潜在靶点变得越来越重要。在这里, 我们提出了一个协议的特普标签 RpdA 结合 HDAC 活性检测, 允许体外疗效测试组蛋白去乙酰化酶抑制剂的方案.

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TAP-taggedHistone DeacetylaseAspergillus NidulansEnzymatic Activity AssayClass 1 Histone DeacetylasesRpdAFungal InfectionsHDAC ComplexesFungal-specific Deacetylase InhibitorsConidiaMyceliaLyophilizationBall MillExtraction Buffer

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Cite this Article

Bauer, I., Pidroni, A., Bayram, Ö., Brosch, G., Graessle, S. Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay. J. Vis. Exp. (147), e59527, doi:10.3791/59527 (2019).

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