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CRISPR/Cas9 technologie in het herstellen van Dystrofine expressie in door iPSC afgeleide spier voorlopercellen
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Developmental Biology
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JoVE Journal Developmental Biology
CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors
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CRISPR/Cas9 technologie in het herstellen van Dystrofine expressie in door iPSC afgeleide spier voorlopercellen

Nederlands

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8,172 Views

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07:44 min

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September 14, 2019

DOI:

10.3791/59432-v

DOI:
10.3791/59432-v

•

07:44 min
September 14, 2019

•

7 Views
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1Medical College of Georgia,Augusta University

Summary

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Hier presenteren we een Cas9-gebaseerd exon23 deletie protocol om Dystrofine expressie in iPSC te herstellen van DMDMDX Mouse-afgeleide huid fibroblasten en direct ipscs te onderscheiden in myogene voorlopercellen (MPC) met behulp van het Tet-on myod activeringssysteem.

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Cite this Article

Jin, Y., Shen, Y., Su, X., Weintraub, N., Tang, Y. CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors. J. Vis. Exp. (151), e59432, doi:10.3791/59432 (2019).

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