Journal
/
Medicine
/
一种分离和扩增人类脂肪衍生的美源性干细胞的无酶方法
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells
Please note that all translations are automatically generated. Click here for the English version.

一种分离和扩增人类脂肪衍生的美源性干细胞的无酶方法

English

Automatically Generated

9,900 Views

•

04:37 min

•

December 16, 2019

DOI:

10.3791/59419-v

DOI:
10.3791/59419-v

•

04:37 min
December 16, 2019

•

42 Views
, , , ,
1Department of Medicine, Division of Hematology/Oncology, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Rutgers School of Graduate Studies at New Jersey Medical School,Rutgers Biomedical and Health Sciences, 3Department of Surgery, Division of Plastic Surgery, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Summary

Automatically generated

该协议提供了一种无酶方法，用于使用外植方法从腹肌成形和脂质酶样本中分离中质性干细胞。没有苛刻的酶或离心步骤提供了临床相关的干细胞，可用于体外研究或转回诊所。

Explore More Videos

Adipose-derived Mesenchymal Stem CellsEnzyme-free IsolationExplant MethodCell ExpansionTrypsinizationClinical ApplicationsResearch Applications

Read Article

Cite this Article

Sherman, L. S., Condé-Green, A., Naaldijk, Y., Lee, E. S., Rameshwar, P. An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells. J. Vis. Exp. (154), e59419, doi:10.3791/59419 (2019).

Download .ris file

Copy

Share Video

.

Copy