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Identificatie van Nucleollaire factoren tijdens de HIV-1-replicatie via Rev-immunoprecipitatie en massaspectrometrie
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Immunology and Infection
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JoVE Journal Immunology and Infection
Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
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Identificatie van Nucleollaire factoren tijdens de HIV-1-replicatie via Rev-immunoprecipitatie en massaspectrometrie

Nederlands

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7,865 Views

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09:38 min

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June 26, 2019

DOI:

10.3791/59329-v

DOI:
10.3791/59329-v

•

09:38 min
June 26, 2019

•

1 Views
, , , , , ,
1Molecular and Cellular Biology Department,Beckman Research Institute at the City of Hope, 2Irell & Manella Graduate School of Biological Sciences, 3Department of Molecular Immunology,Beckman Research Institute and the City of Hope

Summary

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Hier beschrijven we Rev immunoprecipitatie in de aanwezigheid van HIV-1 replicatie voor massaspectrometrie. De beschreven methoden kunnen worden gebruikt voor de identificatie van nucleollaire factoren die betrokken zijn bij de HIV-1-infectieuze cyclus en zijn van toepassing op andere ziekte modellen voor de karakterisering van onderbestudeerde trajecten.

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Arizala, J. A. C., Chomchan, P., Li, H., Moore, R., Ge, H., Ouellet, D. L., Rossi, J. J. Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry. J. Vis. Exp. (148), e59329, doi:10.3791/59329 (2019).

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