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Imaging delle vescibole extracellulari mediante microscopia a forza atomica
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Biochemistry
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JoVE Journal Biochemistry
Imaging of Extracellular Vesicles by Atomic Force Microscopy
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Imaging delle vescibole extracellulari mediante microscopia a forza atomica

italiano

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12,582 Views

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10:11 min

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September 11, 2019

DOI:

10.3791/59254-v

DOI:
10.3791/59254-v

•

10:11 min
September 11, 2019

•

38 Views
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1Department of Chemical Engineering,University of Utah, 2The Nano Institute of Utah,University of Utah, 3Center for Photonics and Quantum Materials,Skolkovo Institute of Science and Technology, 4Biopharmaceutical Cluster ‘Northern’,Moscow Institute of Physics and Technology

Summary

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Viene descritta una procedura passo-passo per l'immobilizzazione senza etichette di esosomi e vescicoli extracellulari da campioni liquidi e la loro imaging mediante microscopia a forza atomica (AFM). Le immagini AFM vengono utilizzate per stimare le dimensioni delle vesciche nella soluzione e caratterizzare altre proprietà biofisiche.

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Cite this Article

Skliar, M., Chernyshev, V. S. Imaging of Extracellular Vesicles by Atomic Force Microscopy. J. Vis. Exp. (151), e59254, doi:10.3791/59254 (2019).

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