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Imaging van extracellulaire vesicles door Atoom kracht microscopie
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Biochemistry
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JoVE Journal Biochemistry
Imaging of Extracellular Vesicles by Atomic Force Microscopy
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Imaging van extracellulaire vesicles door Atoom kracht microscopie

Nederlands

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12,590 Views

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10:11 min

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September 11, 2019

DOI:

10.3791/59254-v

DOI:
10.3791/59254-v

•

10:11 min
September 11, 2019

•

38 Views
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1Department of Chemical Engineering,University of Utah, 2The Nano Institute of Utah,University of Utah, 3Center for Photonics and Quantum Materials,Skolkovo Institute of Science and Technology, 4Biopharmaceutical Cluster ‘Northern’,Moscow Institute of Physics and Technology

Summary

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Een stapsgewijze procedure wordt beschreven voor het label vrij immobilisatie van exosomen en extracellulaire blaasjes uit vloeibare monsters en hun beeldvorming door Atoom kracht microscopie (AFM). De AFM-beelden worden gebruikt om de grootte van de blaasjes in de oplossing te schatten en andere biofysische eigenschappen te karakteriseren.

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Cite this Article

Skliar, M., Chernyshev, V. S. Imaging of Extracellular Vesicles by Atomic Force Microscopy. J. Vis. Exp. (151), e59254, doi:10.3791/59254 (2019).

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