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Estudando o RNA interactianos de proteína quinase RNA-ativado durante o ciclo celular dos mamíferos
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Biochemistry
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JoVE Journal Biochemistry
Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Estudando o RNA interactianos de proteína quinase RNA-ativado durante o ciclo celular dos mamíferos

português

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6,304 Views

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10:05 min

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March 05, 2019

DOI:

10.3791/59215-v

DOI:
10.3791/59215-v

•

10:05 min
March 05, 2019

•

5 Views
, ,
1Department of Chemical and Biomolecular Engineering and KI for Health Science and Technology (KIHST),Korea Advanced Institute of Science and Technology (KAIST)

Summary

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Nós apresentamos abordagens experimentais para estudar RNA-interactianos de double-stranded RNA ligação proteína quinase RNA-ativado (PKR) durante o ciclo de pilha mamífero usando células HeLa. Este método utiliza o formaldeído para complexos de crosslink RNA-PKR e imunoprecipitação para enriquecer RNAs ligados a PKR. Estes RNAs podem ser analisados ainda mais através de sequenciamento de alto rendimento ou qRT-PCR.

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Cite this Article

Kim, S., Kang, M., Kim, Y. Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle. J. Vis. Exp. (145), e59215, doi:10.3791/59215 (2019).

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