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Un dispositivo de posicionamiento para la colocación de ratones durante la entrega de siRNA intranasal al sistema nervioso central
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System
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Un dispositivo de posicionamiento para la colocación de ratones durante la entrega de siRNA intranasal al sistema nervioso central

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21,977 Views

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05:28 min

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August 15, 2019

DOI:

10.3791/59201-v

DOI:
10.3791/59201-v

•

05:28 min
August 15, 2019

•

43 Views
, , , ,
1Department of Bioengineering and Institute of Nanoscience and Technology,Hanyang University, 2Department of Internal Medicine, Section of Infectious Diseases,Yale University School of Medicine

Summary

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Aquí, presentamos un protocolo utilizando un dispositivo de posicionamiento del ratón que permite la colocación adecuada de ratones para la administración intranasal de una formulación de péptido-siRNA dirigida al cerebro que permite un silenciamiento eficaz del gen en el sistema nervioso central.

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Ullah, I., Chung, K., Beloor, J., Lee, S., Kumar, P. A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System. J. Vis. Exp. (150), e59201, doi:10.3791/59201 (2019).

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