Journal
/
Biochemistry
/
Preparación de hongos y materiales vegetales para la elucidación estructural mediante RMN de estado sólido de polarización Nuclear dinámica
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
Please note that all translations are automatically generated. Click here for the English version.

Preparación de hongos y materiales vegetales para la elucidación estructural mediante RMN de estado sólido de polarización Nuclear dinámica

español

Automatically Generated

7,299 Views

•

09:37 min

•

February 12, 2019

DOI:

10.3791/59152-v

DOI:
10.3791/59152-v

•

09:37 min
February 12, 2019

•

8 Views
, , , , ,
1Department of Chemistry,Louisiana State University, 2National High Magnetic Field Laboratory, 3Departments of Pediatrics, and Microbiology, Immunology and Parasitology,Louisiana State University Health Sciences Center

Summary

Automatically generated

Se presenta un protocolo para preparar 13C,15marcado con N muestras de hongos y plantas para espectroscopia de RMN de estado sólida multidimensional y las investigaciones de la polarización nuclear dinámica (DNP).

Explore More Videos

Fungal MaterialsPlant MaterialsSolid-state NMRDynamic Nuclear PolarizationBiomaterialsCarbohydratesFungal Cell WallsCell CultureYPD MediumLabeled Minimal MediumIsotope LabelingCarbon-13Nitrogen-15SterilizationAutoclaveCell Culture Techniques

Read Article

Cite this Article

Kirui, A., Dickwella Widanage, M. C., Mentink-Vigier, F., Wang, P., Kang, X., Wang, T. Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR. J. Vis. Exp. (144), e59152, doi:10.3791/59152 (2019).

Download .ris file

Copy

Share Video

.

Copy