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破骨細胞形成と培養破骨細胞前駆体の増殖に及ぼす抗 c-fms 抗体
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Immunology and Infection
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JoVE Journal Immunology and Infection
Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro
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破骨細胞形成と培養破骨細胞前駆体の増殖に及ぼす抗 c-fms 抗体

日本語

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5,846 Views

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07:51 min

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March 18, 2019

DOI:

10.3791/59089-v

DOI:
10.3791/59089-v

•

07:51 min
March 18, 2019

•

14 Views
, , , , , , , , , , ,
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine,Tohoku University Graduate School of Dentistry

Summary

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このプロトコルにはマウス長管骨および骨髄分離、骨髄のマクロファージを生成し、破骨細胞マクロファージコロニー刺激因子 (M-CSF) と核因子 κ B ligand (RANKL) の受容体活性化剤を使用するが含まれていますか腫瘍壊死因子 α (TNF-α) と M-CSF 受容体抗体抗 c fms によって彼らの逮捕。

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Cite this Article

Marahleh, A., Kitaura, H., Ishida, M., Shima, K., Kishikawa, A., Ogawa, S., Shen, W., Qi, J., Ohori, F., Noguchi, T., Nara, Y., Mizoguchi, I. Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro. J. Vis. Exp. (145), e59089, doi:10.3791/59089 (2019).

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