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Effect van Anti-c-fms antilichaam op Osteoclast vorming en verspreiding van Osteoclast voorloper In Vitro
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Immunology and Infection
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JoVE Journal Immunology and Infection
Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro
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Effect van Anti-c-fms antilichaam op Osteoclast vorming en verspreiding van Osteoclast voorloper In Vitro

Nederlands

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5,846 Views

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07:51 min

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March 18, 2019

DOI:

10.3791/59089-v

DOI:
10.3791/59089-v

•

07:51 min
March 18, 2019

•

14 Views
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1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine,Tohoku University Graduate School of Dentistry

Summary

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Dit protocol omvat dissectie van lymfkliertest lange botten en beenmerg isolatie, voor het genereren van beenmerg macrofagen en produceren van de osteoclasten met behulp van macrofaag kolonie stimulerende factor (M-CSF) en receptor activator van nuclear factor-Kappa-B ligand (RANKL) of Tumornecrosefactor alfa (TNF-α) en hun arrestatie door anti-c-fms antilichaam, M-CSF receptor.

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Cite this Article

Marahleh, A., Kitaura, H., Ishida, M., Shima, K., Kishikawa, A., Ogawa, S., Shen, W., Qi, J., Ohori, F., Noguchi, T., Nara, Y., Mizoguchi, I. Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro. J. Vis. Exp. (145), e59089, doi:10.3791/59089 (2019).

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