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プロテアーゼ活性の画面に蛍光ペプチド胸の谷間アッセイ: アプリケーション コロナ ウイルスのスパイク蛋白質のアクティブ化
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
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プロテアーゼ活性の画面に蛍光ペプチド胸の谷間アッセイ: アプリケーション コロナ ウイルスのスパイク蛋白質のアクティブ化

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07:53 min

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January 09, 2019

DOI:

10.3791/58892-v

DOI:
10.3791/58892-v

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07:53 min
January 09, 2019

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1Department of Microbiology and Immunology, College of Veterinary Medicine,Cornell University, 2Department of Microbiology, College of Agricultural and Life Sciences,Cornell University, 3Virologie et Immunologie Moléculaires, Domaine de Vilvert,INRA, 4Department of Cell Biology and Molecular Genetics,University of Maryland

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Fluorogenic Peptide Cleavage AssayProteolytic ActivityCoronavirus Spike Protein ActivationProteolytic ModificationProtease CharacterizationViral FusionPeptide DesignFRET PairFluorescence Plate ReaderProtease Activity Assay

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Jaimes, J. A., Millet, J. K., Goldstein, M. E., Whittaker, G. R., Straus, M. R. A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation. J. Vis. Exp. (143), e58892, doi:10.3791/58892 (2019).

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